An Optogenetic Platform for Real-Time, Single-Cell Interrogation of Stochastic Transcriptional Regulation
نویسندگان
چکیده
منابع مشابه
A stochastic transcriptional switch model for single cell imaging data
Gene expression is made up of inherently stochastic processes within single cells and can be modeled through stochastic reaction networks (SRNs). In particular, SRNs capture the features of intrinsic variability arising from intracellular biochemical processes. We extend current models for gene expression to allow the transcriptional process within an SRN to follow a random step or switch funct...
متن کاملReal-Time Phase Masks for Interactive Stimulation of Optogenetic Neurons
Experiments with networks of optogenetically altered neurons require stimulation with high spatio-temporal selectivity. Computer-assisted holography is an energy-efficient method for robust and reliable addressing of single neurons on the millisecond-timescale inherent to biologial information processing. We show that real-time control of neurons can be achieved by a CUDA-based hologram computa...
متن کاملan application of fuzzy logic for car insurance underwriting
در ایران بیمه خودرو سهم بزرگی در صنعت بیمه دارد. تعیین حق بیمه مناسب و عادلانه نیازمند طبقه بندی خریداران بیمه نامه براساس خطرات احتمالی آنها است. عوامل ریسکی فراوانی می تواند بر این قیمت گذاری تاثیر بگذارد. طبقه بندی و تعیین میزان تاثیر گذاری هر عامل ریسکی بر قیمت گذاری بیمه خودرو پیچیدگی خاصی دارد. در این پایان نامه سعی در ارائه راهی جدید برای طبقه بندی عوامل ریسکی با استفاده از اصول و روش ها...
Multiarray cell stretching platform for high-magnification real-time imaging.
AIM This article reports the development of a multiarray microchip with real-time imaging capability to apply mechanical strains onto monolayered cell cultures. MATERIALS & METHODS Cells were cultured on an 8-µm thick membrane that was positioned in the microscope focal plane throughout the stretching process. Each stretching unit was assembled from three elastomeric layers and a glass covers...
متن کاملFunctional Interrogation of an Odorant Receptor Locus Reveals Multiple Axes of Transcriptional Regulation
The odorant receptor (OR) genes constitute the largest mammalian gene family and are expressed in a monogenic and monoallelic fashion, through an unknown mechanism that likely exploits positive and negative regulation. We devised a genetic strategy in mice to examine OR selection by determining the transcriptional activity of an exogenous promoter homologously integrated into an OR locus. Using...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Cell
سال: 2018
ISSN: 1097-2765
DOI: 10.1016/j.molcel.2018.04.012